Preoperative localization of single gland disease in primary hyperparathyroidism: a comparative study for preoperative localization with four-dimensional computed tomography, ultrasonography, and technetium-99m-sestamibi single-photon emission computed tomography
Highlight box
Key findings
• Four-dimensional computed tomography (4D CT), neck ultrasonography (US), and technetium-99m-sestamibi single-photon emission computed tomography (99mTc-sestamibi SPECT) are valuable for localizing parathyroid adenoma(s) in primary hyperparathyroidism (PHPT).
• Among the localization modalities, 4D CT demonstrated the highest sensitivity and specificity.
What is known and what is new?
• US and 99mTc-sestamibi SPECT have been widely used for preoperative localization in patients with PHPT; however, each has limitations, particularly in cases with concurrent thyroid pathology or smaller parathyroid tumors.
• 4D CT outperformed US and 99mTc-sestamibi SPECT in sensitivity and specificity for PHPT localization, maintaining accuracy even when US and 99mTc-sestamibi SPECT yield negative results.
• While previous studies have compared these modalities individually, only few have directly compared all three together. Therefore, this study evaluated all three imaging modalities simultaneously, reaffirming the clear diagnostic advantages of 4D CT and providing a comprehensive comparison that may guide optimal imaging selection in clinical practice.
What is the implication, and what should change now?
• Given the superior localization accuracy of 4D CT, especially in challenging cases, it can be actively considered as a primary imaging modality for PHPT when US and 99mTc-sestamibi SPECT are inconclusive.
Introduction
Primary hyperparathyroidism (PHPT) is characterized by the excessive secretion of parathyroid hormone (PTH), which typically results in hypercalcemia. In 85–90% of cases, a single benign parathyroid adenoma is the underlying cause, while carcinoma is extremely rare, accounting for less than 1% (1,2). PHPT, diagnosed based on clinical and biochemical criteria with elevated serum calcium and PTH levels, can lead to several complications, including increased risk of fracture due to osteoporosis, nephrolithiasis, cardiovascular disease, and peptic ulcer disease (2). The mainstay of treatment for PHPT is parathyroidectomy, and the indications for surgery are well-described in the latest guidelines (3).
To ensure successful parathyroidectomy, precise localization of the abnormal parathyroid gland via thorough imaging is essential. This has enabled the adoption of minimally invasive parathyroidectomy as an alternative to the previously favored bilateral neck exploration, which was usually performed in the past. Although a recent systematic review did not conclusively show that minimally invasive parathyroidectomy is superior to bilateral neck exploration (4), it, including unilateral open parathyroidectomy, offers clear advantages in terms of reduced surgical risks and hospitalization, lower rates of complications, and lower patient costs (5-7).
Various methods have been proposed for the localization of the parathyroid adenoma(s) in patients with PHPT (8-10). According to current treatment guidelines for PHPT, high-resolution neck ultrasonography (US), integrated technetium-99m-sestamibi single-photon-emission computed tomography (99mTc-sestamibi SPECT), and contrast-enhanced four-dimensional computed tomography (4D CT) are recommended modalities for imaging evaluation (3). Therefore, this study compared the sensitivity and accuracy of these three imaging modalities for the evaluation of patients with single gland disease and assessed the usefulness of 4D CT compared with US and 99mTc-sestamibi SPECT. We present this article in accordance with the STARD reporting checklist (available at https://gs.amegroups.com/article/view/10.21037/gs-2024-482/rc).
Methods
Patients
A total of 41 patients who underwent surgery for single gland disease between January 2021 and March 2023 were enrolled in this study. All patients underwent surgery for the first time and were diagnosed with PHPT based on elevated blood PTH and calcium levels. Laboratory values, including blood PTH and calcium levels, were collected for all patients up to six months postoperatively. Preoperative imaging for all patients included neck US, 99mTc-sestamibi SPECT, and 4D CT. Patients diagnosed with single gland disease using all three imaging modalities and subsequently confirmed through postoperative pathology results were included in this study. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). This retrospective study was approved by the Institutional Review Board of by the Pusan National University Hospital (No. 2302-010-124), and the requirement for informed consent was waived owing to the retrospective nature of the study.
High-resolution neck US
US examinations were performed by three endocrinologists with over 10 years of experience, using a US machine with a 7.5–15-MHZ linear transducer (Acuson S2000, SIEMENS Healthineers, Erlangen, Bayern, Germany; or Aplio i700, Canon Medical Systems, Otawara, Tochigi, Japan). The US was conducted first as part of the preoperative evaluation to ensure that the endocrinologists were blinded to the results of other imaging modalities. US examinations were performed with the patient in the supine position with the neck extended. Concomitant thyroid lesions (thyroiditis, multinodular goiter, or huge goiter) were evaluated simultaneously. Parathyroid lesions were characterized as abnormal when a well-circumscribed or slightly lobulated homogeneously echogenic mass was present in the expected area. When necessary, abnormal parathyroid glands were further evaluated using Doppler imaging, which showed polar vessels or rim-pattern vascularity.
99mTc-sestamibi SPECT
Dual-phase acquisitions of planar imaging, consisting of the anterior neck and upper thorax, were conducted at 20 minutes (early imaging) and 120 minutes (delayed imaging) after intravenous administration of a dose of 925 MBq (25 mCi) of 99mTc-sestamibi (Jubilant Radiopharma, Philadelphia, Pennsylvania, USA). The patients were placed in the supine position with their necks extended and their arms lowered alongside their bodies. Planar images were obtained using a dual-detector gamma camera (Symbia, SIEMENS Healthineers, Erlangen, Bayern, Germany), with static mode images obtained using a 256×256-pixel matrix and a 1.0× zoom factor at the previously mentioned predetermined time points (20 and 120 minutes). SPECT imaging was completed using a 128×128-pixel matrix, 1.0× zoom factor, and a slice thickness of 4.42 mm. Increased focal uptake and prolonged retention of 99mTc-sestamibi on a delayed 120-minute image was considered a positive finding for parathyroid lesions (11,12). All 99mTc-sestamibi SPECT images were interpreted by a board-certified nuclear medicine physician with over 10 years of experience in nuclear imaging, who was blinded to the results of other imaging modalities when interpreting the SPECT scans.
Contrast-enhanced 4D CT
Patients underwent 4D CT on a 64-row multirow detector CT scanner (Revolution Frontier, Revolution Apex, or Discovery CT 750 HD; GE HealthCare, Chicago, Illinois, USA). The images were reconstructed in the axial plane with a thickness of 1.25 mm. Nonenhanced images were obtained prior to injection of the contrast medium. After intravenous injection of 100 mL of contrast media, arterial, venous, and delayed-phase images were acquired at 30, 60, and 80 s, respectively. The 4D CT enhancement patterns (types A, B, and C), which indicate abnormal parathyroid glands, were classified according to the criteria described in a previous study (13). All 4D CT images were interpreted by a single radiologist with over 5 years of experience, who was blinded to the results of other imaging modalities. We then analyzed the clinical and pathological parameters according to the pattern of enhancement.
Surgery
Minimally invasive parathyroidectomy via a lateral approach or unilateral open parathyroidectomy was performed by two head and neck surgeons with >5 years of experience. The location of the abnormal parathyroid lesion, removed during surgery and confirmed pathologically, was used as the reference standard. Intraoperative frozen section biopsy was performed to confirm that the removed tissue was a parathyroid gland, and a subsequent pathologic confirmation was determined, diagnosing parathyroid adenoma, hyperplasia, or carcinoma. We obtained the dimensions of each parathyroid gland (length, width, and thickness) from the pathologic reports and calculated the corresponding semiaxes (a, b, and c). The gland volume was subsequently estimated using the ellipsoid volume formula: . All patients were followed for 6 months postoperatively.
Statistical analysis
Statistical analyses were performed using R version 4.2.2 (R Development Core Team, http://cran.r-project.org/). A normality test was conducted to assess the distribution of continuous variables prior to performing statistical comparisons. The characteristics of parathyroid lesions were compared among the three relative enhancement patterns on 4D CT. For these comparisons, continuous variables were analyzed using either one-way analysis of variance (ANOVA) or the Kruskal-Wallis test, depending on whether or not normality is satisfied. In contrast, categorical data were analyzed using the Chi-squared or Fisher’s exact test. When comparing the two groups, continuous variables were analyzed using an independent t-test or Wilcoxon rank-sum test, whereas categorical data were analyzed using Fisher’s exact test. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of the three imaging modalities were determined based on the reference standard location. The 95% confidence intervals (CIs) for accuracy were calculated using the Clopper-Pearson exact method. Statistical significance was defined as P<0.05.
Results
Patients
Table 1 summarizes the demographic and clinical characteristics of the 41 patients who underwent surgery and were enrolled in this study. Thyroid surgery was performed concurrently in 12 patients. All patients who underwent concomitant surgery had coexisting conditions, including micropapillary thyroid cancer or large thyroid nodules. All parathyroid lesions were confirmed to be benign, with 28 cases identified as parathyroid adenoma and 13 as parathyroid hyperplasia. Blood PTH and calcium levels decreased postoperatively in all patients and were confirmed to be within the normal range at 6 months. None of them experienced postoperative vocal cord paralysis. Detailed information on the localization accuracy of US, 4D CT, and SPECT for all 41 cases, compared with surgical findings, is provided in Table S1.
Table 1
Characteristics | Value (n=41) |
---|---|
Age (years) | 58.9±10.1 [34–81] |
Gender | |
Male | 6 (14.6) |
Female | 35 (85.4) |
Tumor | |
Size (cm) | 1.60±0.64 |
Volume (cm3) | 5.60±5.16 |
Preoperative test | |
PTH (pg/mL) | 153.9±110.7 |
Calcium (mg/dL) | 11.4±1.1 |
DXA T-score | −1.4±1.6 |
Location of abnormal parathyroid | |
Right | |
Upper | 6 (14.6) |
Lower | 10 (24.4) |
Left | |
Upper | 14 (34.2) |
Lower | 11 (26.8) |
Data were presented as mean ± standard deviation [range] or n (%). DXA, dual-energy X-ray absorptiometry; PTH, parathyroid hormone.
Comparison of 4D CT, US, and 99mTC-sestamibi SPECT
The sensitivity, specificity, and accuracy of the three imaging modalities were as follows: 4D CT, 95.1%, 98.4%, and 97.6%; US, 82.9%, 95.1%, and 92.1%; and 99mTc-sestamibi SPECT, 78.0%, 97.6%, and 92.7%, respectively (Table 2). Although 4D CT was unable to accurately determine abnormal parathyroid lesions in two cases out of the total, the sensitivity, specificity, and accuracy of 4D CT were the highest of the three imaging modalities.
Table 2
Predictive performance values | 4D CT | US | 99mTc-sestamibi SPECT | |||||
---|---|---|---|---|---|---|---|---|
Rate | 95% CI | Rate | 95% CI | Rate | 95% CI | |||
Sensitivity (%) | 95.1 | 83.5–99.4 | 82.9 | 67.9–92.8 | 78.0 | 62.4–89.4 | ||
Specificity (%) | 98.4 | 94.2–99.8 | 95.1 | 89.7–98.2 | 97.6 | 93.0–99.5 | ||
PPV (%) | 95.1 | 83.5–99.4 | 85.0 | 70.2–94.3 | 91.4 | 76.9–98.2 | ||
NPV (%) | 98.4 | 94.2–99.8 | 94.4 | 88.7–97.7 | 93.0 | 87.2–96.8 | ||
Accuracy (%) | 97.6 | 93.9–99.3 | 92.1 | 86.8–95.7 | 92.7 | 87.6–96.2 |
4D CT, four-dimensional computed tomography; 99mTc-sestamibi SPECT, technetium-99m-sestamibi single-photon emission computed tomography; CI, confidence interval; NPV, negative predictive value; PPV, positive predictive value; US, ultrasonography.
We also analyzed the combined performance of the imaging modalities. The sensitivity, specificity, and accuracy for the combined modalities were as follows: US + 99mTc-sestamibi SPECT demonstrated 95.1%, 98.4%, and 97.6%, respectively; and 99mTc-sestamibi SPECT + 4D CT yielded 97.6%, 99.2%, and 98.8%, respectively (Table 3). Additionally, in the case of US + 99mTc-sestamibi SPECT, the localization results were found to be identical to those obtained when 4D CT was used alone (Tables 2,3). For US + 4D CT, correct localization of parathyroid adenomas was achieved in all 41 patients. Similarly, when all three imaging modalities (US + 99mTc-sestamibi SPECT + 4D CT) were used together, 100% accuracy was observed across all measures (Table 3).
Table 3
Predictive performance values | US + 99mTc-sestamibi SPECT | 4D CT + 99mTc-sestamibi SPECT | US + 4D CT | US + 4DCT + 99mTc-sestamibi SPECT |
---|---|---|---|---|
Sensitivity (%) | 95.1 | 97.6 | 100 | 100 |
Specificity (%) | 98.4 | 99.2 | 100 | 100 |
PPV (%) | 95.1 | 97.6 | 100 | 100 |
NPV (%) | 98.4 | 99.2 | 100 | 100 |
Accuracy (%) | 97.6 | 98.8 | 100 | 100 |
4D CT, four-dimensional computed tomography; 99mTc-sestamibi SPECT, technetium-99m-sestamibi single-photon emission computed tomography; NPV, negative predictive value; PPV, positive predictive value; US, ultrasonography.
Based on the enhancement patterns of parathyroid lesions using 4D CT, 19 cases (46.3%) were classified as type A, 20 cases (48.8%) as type B, and 2 cases (4.9%) as type C (Table 4). No comparison was observed between enhancement type and tumor volume, false-positive lesions, or parathyroid lesion location.
Table 4
Characteristics | Type A (n=19) | Type B (n=20) | Type C (n=2) | P value |
---|---|---|---|---|
Age (years) | 60.2±10.4 | 58.3±10.0 | 52.5±9.2 | 0.50 |
Gender | >0.99 | |||
Male | 3 (15.8) | 3 (15.0) | 0 | |
Female | 16 (84.2) | 17 (85.0) | 2 (100.0) | |
Tumor | ||||
Size (cm) | 1.64±0.66 | 1.56±0.63 | 1.65±0.92 | 0.78 |
Volume (cm3) | 4.73±4.76 | 6.03±5.05 | 9.55±11.01 | 0.84 |
False positive lesion | 0 | 2 | 0 | 0.54 |
Preoperative test | ||||
PTH (pg/mL) | 164.1±136.5 | 140.2±77.3 | 193.7±180.3 | 0.78 |
Calcium (mg/dL) | 11.3±1.4 | 11.5±0.7 | 12.0±0.7 | 0.10 |
Location of parathyroid lesion | 0.61 | |||
Right | ||||
Upper | 1 (5.3) | 5 (25.0) | 0 | |
Lower | 5 (26.3) | 5 (25.0) | 0 | |
Left | ||||
Upper | 8 (42.1) | 6 (30.0) | 0 | |
Lower | 5 (26.3) | 4 (20.0) | 2 (100.0) |
Data were presented as mean ± standard deviation, n (%), or n. PTH, parathyroid hormone.
Incorrect localization cases in US and false negatives cases in 99mTc-sestamibi SPECT
Nine of the 41 cases examined using US exhibited incorrect localization. Preoperative PTH levels, tumor volume, and tumor location were not associated with incorrect localization. However, the presence of coexisting thyroid lesions, such as thyroiditis, multinodular goiter, or large thyroid nodule, was significantly associated with incorrect localization on US (P=0.03). Notably, all nine cases that showed incorrect localization on US were accurately localized on 4D CT (Table 5, Figure 1).
Table 5
Characteristics | US incorrect (n=9) | US correct (n=32) | P value |
---|---|---|---|
Age (years) | 58.8±5.4 | 58.9±11.1 | 0.96 |
Gender | >0.99 | ||
Male | 1 (11.1) | 5 (15.6) | |
Female | 8 (88.9) | 27 (84.4) | |
Tumor | |||
Size (cm) | 1.67±0.47 | 1.58±0.69 | 0.73 |
Volume (cm3) | 5.86±5.61 | 5.53±5.11 | 0.87 |
Preoperative test | |||
PTH (pg/mL) | 130.2±88.3 | 160.5±116.6 | 0.48 |
Calcium (mg/dL) | 11.7±1.0 | 11.4±1.1 | 0.38 |
Coexisting thyroid lesion | 0.03* | ||
Presence | 7 (77.8) | 11 (34.4) | |
Absence | 2 (22.2) | 21 (65.6) | |
Location of parathyroid lesion | 0.69 | ||
Right | |||
Upper | 2 (22.2) | 4 (12.5) | |
Lower | 1 (11.1) | 9 (28.1) | |
Left | |||
Upper | 4 (44.4) | 10 (31.2) | |
Lower | 2 (22.2) | 9 (28.1) | |
Localization of 4D CT | >0.99 | ||
Correct | 9 (100.0) | 30 (93.8) | |
Incorrect | 0 | 2 (6.2) |
Data were presented as mean ± standard deviation or n (%). *, P<0.05. 4D CT, four-dimensional computed tomography; PTH, parathyroid hormone; US, ultrasonography.

In 99mTc-sestamibi SPECT, 9 out of 41 cases showed false negatives. No significant association was found between preoperative PTH levels or location and false-negative results. However, it is worth noting that tumor volumes were statistically significantly smaller among patients with false negative results compared to those with true positive results (P=0.008). Notably, eight of the nine cases that showed false negatives on 99mTc-sestamibi SPECT were successfully localized on 4D CT (Table 6, Figure 2).
Table 6
Characteristics | 99mTc-sestamibi SPECT incorrect (n=9) | 99mTc-sestamibi SPECT correct (n=32) | P value |
---|---|---|---|
Age (years) | 57.0±12.4 | 59.4±9.5 | 0.53 |
Gender | >0.99 | ||
Male | 1 (11.1) | 5 (15.6) | |
Female | 8 (88.9) | 27 (84.4) | |
Tumor | |||
Size (cm) | 1.23±0.56 | 1.70±0.63 | 0.051 |
Volume (cm3) | 2.69±2.69 | 6.42±5.41 | 0.008* |
Preoperative test | |||
PTH (pg/mL) | 172.7±174.6 | 148.6±88.5 | 0.70 |
Calcium (mg/dL) | 10.8±1.1 | 11.6±1.0 | 0.04* |
Location of parathyroid lesion | 0.31 | ||
Right | |||
Upper | 2 (22.2) | 4 (12.5) | |
Lower | 1 (11.1) | 9 (28.1) | |
Left | |||
Upper | 5 (55.6) | 9 (28.1) | |
Lower | 1 (11.1) | 10 (31.3) | |
Localization of 4D CT | 0.40 | ||
Correct | 8 (88.9) | 31 (96.9) | |
Incorrect | 1 (11.1) | 1 (3.1) |
Data were presented as mean ± standard deviation or n (%). *, P<0.05. 4D CT, four-dimensional computed tomography; 99mTc-sestamibi SPECT, technetium-99m-sestamibi single-photon emission computed tomography; PTH, parathyroid hormone.

Of the two cases examined using 4D CT that exhibited incorrect localization, one was accurately localized on 99mTc-sestamibi SPECT. Notably, both cases were correctly localized on US (Tables 5,6).
Discussion
The imaging modalities utilized for the evaluation of PHPT include high-resolution US, integrated 99mTc-sestamibi SPECT, and 4D CT, alongside other modalities such as magnetic resonance imaging of the neck, integrated 18F-choline/11C-methionine positron emission tomography, angiographic selective venous sampling, and various combinations of sestamibi and thyroid scans (8-10). These combinations include sestamibi scans with I-123 thyroid scans with pertechnetate thyroid scans, either alone or with SPECT or SPECT/CT of the neck. These imaging modalities are used complementarily to maximize accuracy and precision in localizing parathyroid adenoma(s) through concordant imaging results (10). Among these methods, 4D CT, introduced in 2006, is one of the most recent (14). The characteristic findings of parathyroid adenoma on 4D CT are lower attenuation than those of the thyroid gland in the non-contrast enhancement phase, higher enhancement than the thyroid gland in the arterial phase, and faster washout than the thyroid gland in the delayed phase (15-17). However, this characteristic enhancement pattern is not the same for all abnormal parathyroid lesions (13).
Various methods have been proposed for the localization of the parathyroid gland in patients with PHPT, but the most commonly used methods are high-resolution neck US, 99mTc-sestamibi SPECT, and 4D CT (3). In a meta-analysis comparing these three imaging methods, the sensitivity and PPV were as follows: US, 76.1% (95% CI: 70.4–81.4%) and 93.2% (95% CI: 90.7–95.3%), respectively; 99mTc-sestamibi SPECT, 78.9% (95% CI: 64–90.6%) and 90.7% (95% CI: 83.5–96.0%), respectively; and 4D CT, 89.4% and 93.5%, respectively (18). In this study, the sensitivity and PPV of 4D CT were higher than those of US and 99mTc-sestamibi SPECT, consistent with the results of the aforementioned meta-analysis (18,19).
The sensitivity of CT used for the localization of parathyroid glands in another study was 73% (20). In subgroup analysis, the sensitivity of CT was higher when adding an additional contrast phase image rather than a single contrast phase (20). The 4D CT (three contrast phases with a non-contrast phase) performed in this study increased the accuracy of abnormal parathyroid localization compared to 2-phase CT with only one contrast phase; however, compared to 2-phase CT, 4D CT has the disadvantage of increasing a patient’s exposure to ionizing radiation (20-22). Although 4D CT has relatively high radiation exposure, the risk of cancer due to 4D CT-related radiation exposure is very low (22). Although this study utilized the 4-phase protocol for 4D CT due to the undetermined optimal number of post-contrast phases, the 3-phase protocol is currently the most commonly used approach (10). Previous studies have demonstrated that the 3-phase protocol achieves comparable diagnostic accuracy while reducing radiation exposure compared to the 4-phase protocol (8-10,15,20).
The 4D CT enhancement patterns for pathological parathyroid lesions can be classified into three previously described types (13). In our study, there were 19 type A cases (46.3%), 20 type B cases (48.8%), and 2 type C cases (4.9%). These results are somewhat different from those of a previous report, which reported 20% for type A, 57% for type B, and 22% for type C (13). Evaluating PHPT with 4D CT may have advantages over evaluation with 2-phase CT. In cases where type B pattern enhancement is observed on 4D CT, it can be easily diagnosed using a 2-phase scan. However, in the case of type C patterns, localization may be challenging with a 2-phase CT scan (13).
US is a noninvasive, inexpensive, and rapid method for tumor localization. It also has the advantage of being able to guide fine-needle aspiration or core biopsies of indeterminate lesions without exposing the patients to ionizing radiation (9,12). However, it has the disadvantage of being operator-dependent, and its sensitivity may decrease in the case of deep or hidden anatomical regions, exophytic thyroid nodules, or large multinodular goiter (8,9). In this study, the sensitivity, specificity, and accuracy of US were 82.9%, 95.1%, and 92.1%, respectively. Notably, a particularly high rate of incorrect localization has been observed in patients with concurrent thyroid lesions. Preoperative localization of the abnormal parathyroid gland was possible on 4D CT in all nine cases that were incorrectly diagnosed with US.
99mTc-sestamibi SPECT has the advantage of being less operator-dependent than US. It also has the advantage of excellent localization of ectopic parathyroid glands (8,9). However, false negatives may occur in cases with small parathyroid lesions or different washout times for parathyroid lesions (11,23). In our study, the sensitivity, specificity, and accuracy of 99mTc-sestamibi SPECT were 78.0%, 97.6%, and 92.7%, respectively, which are consistent with the results of other studies (24-26). There were nine cases in which 99mTc-sestamibi SPECT failed to localize abnormal parathyroid lesions, with false negatives significantly increasing for tumors with smaller volumes. This finding aligns with previous studies and can be attributed to the functional limitations of 99mTc-sestamibi SPECT. Specifically, its lower spatial resolution and reduced sensitivity to low radiotracer uptake in smaller or less metabolically active lesions likely contribute to its diminished performance in identifying small-volume lesions (8,12,23,27).
However, in eight of these nine cases, abnormal parathyroid gland localization was possible with 4D CT. Suh et al. (24) reported that the false negatives of 4D CT were significantly higher when the parathyroid tumor volume was smaller; however, in this study, precise preoperative localization was possible on 4D CT, even in the cases of small-size parathyroid lesions that were not detected on 99mTc-sestamibi SPECT. Among the methods for the preoperative localization of abnormal parathyroid lesions, US and parathyroid scintigraphy are the most common (12,15). We consider the two main disadvantages of 4D CT to be the risks associated with radiation exposure and the use of iodinated contrast media. The use of iodinated contrast media can induce allergic reactions and also poses a risk of contrast-induced nephropathy, making its use more challenging in patients with renal dysfunction. However, 4D CT has higher sensitivity and accuracy than US and 99mTc-sestamibi SPECT for preoperative localization of abnormal parathyroid lesions (18). Additionally, in our study, in cases of incorrect localization on US or 99mTc-sestamibi SPECT, preoperative localization was possible on 4D CT in most cases. As such, the number of radiologists who want to use 4D CT as a first-line study for parathyroid localization is gradually increasing (15).
There are several limitations to this study. First, its retrospective nature and small sample size limit the statistical power of the findings. As a result, there is potential statistical bias, and no statistically significant differences were observed in the CI analysis. Although including patients with multiglandular disease (MGD) could have increased the sample size, we included only patients with single-gland disease due to the lower incidence of PHPT in South Korea (28). Consequently, the exclusion of MGD cases prevented validation of the efficacy and may have introduced selection bias. Second, the findings are based on data from a single institution, which may restrict the generalizability of the results to other clinical settings or populations. To address this limitation, larger multicenter studies are necessary to validate our findings and provide a more comprehensive understanding of the diagnostic performance of 4D CT. Third, this study utilized the 4-phase protocol for 4D CT, which does not reflect the current standard. The 3-phase protocol is now more commonly adopted as it achieves similar diagnostic accuracy with reduced radiation exposure (8-10,15,20). Future research employing the 3-phase protocol will help align findings with contemporary clinical practices. Fourth, while this study confirms similar findings to prior research (9), it does not present novel results. However, it adds value by simultaneously comparing the performance of three imaging modalities (US, 4D CT, and 99mTc-sestamibi), unlike most previous studies, which have typically compared individual or two modalities. Finally, clinical practice often relies on a combined approach using multiple imaging modalities rather than a single modality. The integration of imaging results through multidisciplinary expert panels is essential for accurate localization and optimal clinical decision-making. Future studies should evaluate the diagnostic value of combining modalities and emphasize the role of expert consensus in improving patient outcomes.
Conclusions
In conclusion, 4D CT demonstrated higher sensitivity and specificity than US and 99mTc-sestamibi SPECT for the preoperative localization of abnormal parathyroid lesions. However, further large-scale, multicenter studies are necessary to validate the diagnostic accuracy of 4D CT and clearly define its role. Despite this, 4D CT serves as a valuable tool for the preoperative evaluation of patients with single gland disease.
Acknowledgments
None.
Footnote
Reporting Checklist: The authors have completed the STARD reporting checklist. Available at https://gs.amegroups.com/article/view/10.21037/gs-2024-482/rc
Data Sharing Statement: Available at https://gs.amegroups.com/article/view/10.21037/gs-2024-482/dss
Peer Review File: Available at https://gs.amegroups.com/article/view/10.21037/gs-2024-482/prf
Funding: This study was supported by
Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://gs.amegroups.com/article/view/10.21037/gs-2024-482/coif). The authors have no conflicts of interest to declare.
Ethical Statement:
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